The Human Thyroid Stimulating Hormone CLIA Kit is a Chemiluminescence Immunoassay (CLIA) intended for the quantitative measurement of human Thyroid Stimulating Hormone (TSH) in serum.
Compatible with the ECL100 Chemiluminescent Immunoassay Analyzer.
For research use only. Not for use in diagnostic procedures.
For lab professional use only.
Manufactured by Epitope Diagnostics, Inc.
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The Human Thyroid Stimulating Hormone CLIA Kit is designed, developed, and produced for the quantitative measurement of human TSH level in serum samples. The TSH assay is a two-site sandwich assay to determination the level of follicle-stimulating hormone.
Assay calibrators, controls, or serum samples are added directly to a reaction vessel together with streptavidin coated magnetic particles and biotinylated anti- TSH polyclonal antibody. The magnetic particles capture the biotin antibody as well as an immune-complex in the form of “magnetic particles–biotin TSH antibody–TSH–acridinium ester TSH antibody”.
Materials bound to the solid beads are held in a magnetic field while unbound materials are washed away. Then trigger solutions are added to the reaction vessel, and light emission is measured with the ECL100 Chemiluminescent Immunoassay Analyzer. The relative light units (RLU) are proportional to the concentration of a TSH in the sample. The amount of analyte in the sample is determined from a stored, multi-point calibration curve and reported in serum TSH concentration.
