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| Method | Sandwich CLIA |
|---|---|
| Sample Type (Matrix) | Serum |
| Sample Volume | 20 µL |
| Species | Human |
| Incubation Time | 15 min |
| Range | 0.200 - 70.0 ng/mL |
| Size | 100 tests |
| Regulatory Status | For Research Use Only. Not for use in diagnostic procedures. |
The Human Squamous Cell Carcinoma Antigen CLIA Kit is a Chemiluminescence Immunoassay (CLIA) intended for the quantitative measurement of human Squamous Cell Carcinoma (SCC) concentrations in serum.
Compatible with the ECL100 Chemiluminescent Immunoassay Analyzer.
For research use only. Not for use in diagnostic procedures.
For lab professional use only.
Manufactured by Epitope Diagnostics, Inc.
Please contact us for a quote.
The Human Squamous cell Carcinoma antigen CLIA Kit is designed, developed, and produced for the quantitative measurement of human SCC levels in serum samples. The assay utilizes a two-site “sandwich” technique with two antibodies that bind to different epitopes of SCC.
Assay calibrators, controls, or serum samples are added directly to a reaction vessel together with magnetic particles antibody. The magnetic particles capture the SCC in the form of “magnetic particles–SCC antibody–SCC–acridinium ester SCC antibody”.
Materials bound to the solid beads are held in a magnetic field while unbound materials are washed away. Then trigger solutions are added to the reaction vessel, and light emission is measured with the ECL100 Chemiluminescent Immunoassay Analyzer. The relative light units (RLU) are proportional to the concentration of a SCC in the sample. The amount of analyte in the sample is determined from a stored, multi-point calibration curve and reported in serum SCC concentration.
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