The Human Growth Hormone CLIA Kit is a Chemiluminescence Immunoassay (CLIA) intended for the quantitative measurement of human growth hormone (hGH) concentration in serum.
Compatible with the ECL100 Chemiluminescent Immunoassay Analyzer.
For research use only. Not for use in diagnostic procedures.
For lab professional use only.
Manufactured by Epitope Diagnostics, Inc.
Please contact us for a quote.
The Human growth hormone CLIA Kit is designed, developed, and produced for the quantitative measurement of human hGH level in serum samples. The assay utilizes a two-site “sandwich” technique with two antibodies that bind to different epitopes of hGH.
Assay calibrators, controls, or serum samples are added directly to a reaction vessel together with streptavidin coated magnetic particles and biotinylated anti-hGH polyclonal antibody. The magnetic particles capture the biotin antibody as well as an immune-complex in the form of “magnetic particles–biotin hGH antibody–hGH–acridinium ester hGH antibody”.
Materials bound to the solid beads are held in a magnetic field while unbound materials are washed away. Then trigger solutions are added to the reaction vessel, and light emission is measured with the ECL100 Chemiluminescent Immunoassay Analyzer. The relative light units (RLU) are proportional to the concentration of hGH in the sample. The amount of analyte in the sample is determined from a stored, multi-point calibration curve and reported in serum hGH concentration.
