Human Follicle-stimulating Hormone CLIA Kit (hFSH)

The Human Follicle-stimulating Hormone CLIA Kit is a Chemiluminescence Immunoassay (CLIA) intended for the quantitative measurement of human hFSH concentration in serum.

Compatible with the ECL100 Chemiluminescent Immunoassay Analyzer.

For research use only. Not for use in diagnostic procedures.

For lab professional use only.

Manufactured by Epitope Diagnostics, Inc.

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Test Principle of the Human Follicle-stimulating Hormone CLIA Kit

The Human Follicle-stimulating Hormone CLIA Kit is designed, developed, and produced for the quantitative measurement of human FSH level in serum samples. The FSH assay is a two-site sandwich assay to determine the level of follicle-stimulating hormone.

Assay calibrators, controls, or serum samples are added directly to a reaction vessel together with magnetic particles antibody. The magnetic particles capture the FSH in the form of “magnetic particles–FSH antibody–FSH–acridinium ester FSH antibody”.

Materials bound to the solid beads are held in a magnetic field while unbound materials are washed away. Then trigger solutions are added to the reaction vessel, and light emission is measured with the ECL100 Chemiluminescent Immunoassay Analyzer. The relative light units (RLU) are proportional to the concentration of a FSH in the sample. The amount of analyte in the sample is determined from a stored, multi-point calibration curve and reported in serum FSH concentration.