Human Ferritin CLIA Kit

SKU SKT-043R Category

Product Specifications

Method: Sandwich CLIA
Sample Type (Matrix): Serum
SAMPLE VOLUME:
50 µL
Species: Human
Incubation time: 10 min
Range: 1.00 - 2000 ng/mL
Size: 100 tests
Regulatory Status: For Research Use Only. Not for use in diagnostic procedures.

Description

The Human Ferritin CLIA Kit is a Chemiluminescence Immunoassay (CLIA) intended for the quantitative measurement of human ferritin concentration in serum.

Compatible with the ECL100 Chemiluminescent Immunoassay Analyzer.

For research use only. Not for use in diagnostic procedures.

For lab professional use only.

Manufactured by Epitope Diagnostics, Inc.

Please contact us for a quote.

Test Principle of the Human Ferritin CLIA Kit

The Human Ferritin CLIA Kit is designed, developed, and produced for the quantitative measurement of human FER levels in serum samples. The assay utilizes a two-site “sandwich” technique with two antibodies that bind to different epitopes of FER.

Assay calibrators, controls, or serum samples are added directly to a reaction vessel together with magnetic particles antibody. The magnetic particles capture the FER in the form of “magnetic particles–FER antibody–FER–acridinium ester FER antibody”.

Materials bound to the solid beads are held in a magnetic field while unbound materials are washed away. Then trigger solutions are added to the reaction vessel, and light emission is measured with the ECL100 Chemiluminescent Immunoassay Analyzer. The relative light units (RLU) are proportional to the concentration of a FER in the sample. The amount of analyte in the sample is determined from a stored, multi-point calibration curve and reported in serum FER concentration.

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