The Human Estradiol CLIA Kit is a Chemiluminescence Immunoassay (CLIA) intended for the quantitative measurement of human estradiol concentration in serum.
Compatible with the ECL100 Chemiluminescent Immunoassay Analyzer.
For research use only. Not for use in diagnostic procedures.
For lab professional use only.
Manufactured by Epitope Diagnostics, Inc.
Please contact us for a quote.
The Human Estradiol CLIA Kit is designed, developed, and produced for the quantitative measurement of human E2 level in serum samples. The assay utilizes the competitive binding mechanism for testing.
The serum samples and releasing assay buffer are added to the sample wells, initiating the first incubation period. After, the calibrators, controls, or sample, monoclonal antibody to E2 labeled with microparticles are mixed and incubated, forming an antigen-antibody immuno-complex. Acridinium ester labeled E2 analog unbound to the sample binds to the magnetically microgranular E2 monoclonal antibody, after precipitation in a magnetic field, the supernatant is decanted, and then a wash cycle is performed to remove any remaining substances that are not bound to the magnetic microbeads. Subsequently, the washed compound is sent into the measurement chamber where trigger solution is automatically added to initiate a chemiluminescence reaction.
The light signal is measured by a photomultiplier as relative light units (RLUs), which is inversely proportional to the concentration of E2 present in the sample. The test result is automatically calculated by the system according to the working curve.
