Human Carcinoembryonic Antigen CLIA Kit (CEA)

The Human Carcinoembryonic Antigen CLIA Kit is a Chemiluminescence Immunoassay (CLIA) intended for the quantitative measurement of human Carcinoembryonic Antigen (CEA) concentration in serum.

Compatible with the ECL100 Chemiluminescent Immunoassay Analyzer.

For research use only. Not for use in diagnostic procedures.

For lab professional use only.

Manufactured by Epitope Diagnostics, Inc.

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Test Principle of the Human Carcinoembryonic Antigen CLIA Kit

The Human Carcinoembryonic Antigen CLIA Kit is designed, developed, and produced for the quantitative measurement of human CEA level in serum samples. The assay utilizes a two-site “sandwich” technique with two antibodies that bind to different epitopes of CEA.

Assay calibrators, controls, or serum samples are added directly to a reaction vessel together with magnetic particles antibody. The magnetic particles capture the CEA in the form of “magnetic particles–CEA antibody–CEA–acridinium ester CEA antibody”.

Materials bound to the solid beads are held in a magnetic field while unbound materials are washed away. Then trigger solutions are added to the reaction vessel, and light emission is measured with the ECL100 Chemiluminescent Immunoassay Analyzer. The relative light units (RLU) are proportional to the concentration of a CEA in the sample. The amount of analyte in the sample is determined from a stored, multi-point calibration curve and reported in serum CEA concentration.