Human Carboxylated Osteocalcin (1-43/49) CLIA Kit

The Human Carboxylated Osteocalcin (1-43/49) CLIA Kit is intended for the quantitative determination of human osteocalcin levels in serum or plasma.

Compatible with the ECL100 Chemiluminescent Immunoassay Analyzer.

For research use only. Not for use in diagnostic procedures.

For lab professional use only.

Manufactured by Epitope Diagnostics, Inc.

Please contact us for a quote.

Test Principle of the Human Carboxylated Osteocalcin (1-43/49) CLIA Kit

The Human Carboxylated Osteocalcin (1-43/49) CLIA Kit is designed, developed, and produced for the quantitative measurement of human osteocalcin level in serum or plasma samples. The assay utilizes a two-site “sandwich” technique with two antibodies that bind to different epitopes of osteocalcin.

Assay calibrators, controls, or samples are added directly to a reaction vessel containing streptavidin coated magnetic particles. Simultaneously, an acridinium ester antibody and a biotin antibody are added. The magnetic particles capture the biotin antibody as well as an immuno complex in the form of “magnetic particles – biotin osteocalcin antibody –osteocalcin – acridinium ester osteocalcin antibody”.

The materials bound to the solid phase are held in a magnetic field while unbound materials are washed away. Then, the trigger solution is added to the reaction vessel and light generated by the reaction is measured with the ECL100 Chemiluminescent Immunoassay Analyzer. The relative light units (RLU) are proportional to the concentration of osteocalcin in the sample. The amount of analyte in the sample is determined from a stored, multi-point calibration curve and reported in serum osteocalcin concentration.