The Cancer Antigen 19-9 CLIA Kit is a Chemiluminescence Immunoassay (CLIA) intended for the quantitative measurement of human CA19-9 in serum.
Compatible with the ECL100 Chemiluminescent Immunoassay Analyzer.
For research use only. Not for use in diagnostic procedures.
For lab professional use only.
Manufactured by Epitope Diagnostics, Inc.
Please contact us for a quote.
The Cancer Antigen 19-9 CLIA Kit is designed, developed, and produced for the quantitative measurement of human CA19-9 level in serum samples. The assay utilizes a two-site “sandwich” technique with two antibodies that bind to different epitopes of CA19-9.
Assay calibrators, controls, or serum samples are added directly to a reaction vessel together with magnetic particles and biotinylated anti-CA19-9 polyclonal antibody. The magnetic particles capture the biotin antibody as well as an immunocomplex in the form of “magnetic particles–biotin CA19-9 antibody–CA19-9–acridinium ester CA19-9 antibody”.
Materials bound to the solid beads are held in a magnetic field while unbound materials are washed away. Then, trigger solutions are added to the reaction vessel and light emission is measured with the ECL100 Chemiluminescent Immunoassay Analyzer. The relative light units (RLU) are proportional to the concentration of a CA19-9 in the sample. The amount of analyte in the sample is determined from a stored, multi-point calibration curve and reported in serum CA19-9 concentration.
