The Hepcidin-25 LC-MS/MS Kit is intended for the quantitative determination of hepcidin in serum.
For research use only. Not for use in diagnostic procedures.
For Laboratory Professional Use Only.
This is the RUO version of Immundiagnostik part number KM4000.
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Test Principle of the Hepcidin-25 LC-MS/MS Kit
A simple solid phase extraction (SPE) in the 96-well format is used for hepcidin-25 enrichment. Following chromatographic separation at moderate pressures, the analyte is measured via tandem mass spectrometry (LC-MS/MS).
A stable isotope-labelled version of the analyte is used as an internal standard to correct sample loss during the sample preparation and matrix effects during ionization.