Fecal Quantitative Occult Blood CLIA Kit

The Fecal Quantitative Occult Blood CLIA Kit is a chemiluminescent immunoassay (CLIA) kit intended for the quantitative determination of human hemoglobin levels in stool.

Compatible with the ECL100 Chemiluminescent Immunoassay Analyzer.

For Research Use Only. Not for use in diagnostic procedures.

For Laboratory Professional Use Only.

Manufactured by Epitope Diagnostics, Inc.

Please contact us for a quote.

Test Principle of the Fecal Quantitative Occult Blood CLIA Kit

This CLIA is designed, developed, and produced for the quantitative measurement of q-FOB levels in stool samples. The assay utilizes a two-site “sandwich” technique with two antibodies that bind to different epitopes of hemoglobin.

Assay calibrators, controls, or samples are added directly to a reaction vessel containing streptavidin-coated magnetic particles. Simultaneously, an acridinium ester antibody and a biotin antibody are added. The magnetic particles capture the biotin antibody and an immunocomplex in the form of “magnetic particles – biotin hemoglobin antibody –hemoglobin – acridinium ester hemoglobin antibody.”

The materials bound to the solid phase are held in a magnetic field while unbound materials are washed away. Then, the trigger solution is added to the reaction vessel, and the light generated by the reaction is measured with the ECL100 Chemiluminescent Immunoassay Analyzer. The relative light units (RLU) are proportional to the concentration of hemoglobin in the sample. The amount of analyte in the sample is determined from a stored, multipoint calibration curve and reported in stool hemoglobin concentration.