Fecal H. pylori Antigen CLIA Kit

The Fecal H. pylori Antigen CLIA Kit is a chemiluminescent immunoassay (CLIA) kit intended for the quantitative determination of Helicobacter pylori (H. pylori) antigen levels in stool.

Compatible with the ECL100 Chemiluminescent Immunoassay Analyzer.

For Research Use Only. Not for use in diagnostic procedures.

For Laboratory Professional Use Only.

Manufactured by Epitope Diagnostics, Inc.

Please contact us for a quote.

Test Principle of the Fecal H. pylori Antigen CLIA Kit

This CLIA is designed, developed, and produced for the quantitative measurement of H. pylori antigen in fecal samples. The assay utilizes a two-site “sandwich” technique with two antibodies that bind to different epitopes of H. pylori antigen.

Assay calibrators, controls, or extracted stool samples are added directly to a reaction vessel. Simultaneously, a biotinylated antibody, a streptavidin-coated magnetic particle, and subsequently, an acridinium ester-conjugated antibody are added to the vessel. The magnetic particles capture the biotin antibody and an immunocomplex in the form of “magnetic particles – biotinylated anti-H. pylori antibody –H. pylori antigen– acridinium ester conjugated anti-H. pylori antigen-antibody.”

The materials bound to the solid phase are held in a magnetic field while unbound materials are washed away. Then, the trigger solution is added to the reaction vessel, and the light generated by the reaction is measured with the ECL100 Chemiluminescent Immunoassay Analyzer. The relative light units (RLU) are proportional to the concentration of H. pylori antigen in the sample. The amount of analyte in the sample is determined from a stored, multi-point calibration curve and reported in fecal H. pylori antigen concentration.