The Fecal Giardia lamblia Antigen CLIA Kit is a chemiluminescent immunoassay (CLIA) kit intended for the quantitative determination of Giardia lamblia antigen levels in stool.
Compatible with the ECL100 Chemiluminescent Immunoassay Analyzer.
FDA Class 2 Exempt. For In Vitro Diagnostic Use.
For Laboratory Professional Use Only.
Manufactured by Epitope Diagnostics, Inc.
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The Fecal Giardia lamblia Antigen CLIA Kit is designed, developed, and produced for the quantitative measurement of Giardia lamblia antigen in stool samples. The assay utilizes a two-site “sandwich” technique with two antibodies that bind to different epitopes of Giardia lamblia.
Assay calibrators, controls, or samples are added directly to a reaction vessel containing streptavidin-coated magnetic particles. An acridinium ester-conjugated antibody and a biotin-conjugated antibody are added. The magnetic particles capture the biotin antibody and an immunocomplex in the form of “magnetic particles – biotin anti-Giardia lamblia antibody – Giardia lamblia – acridinium ester anti-Giardia lamblia antibody.”
The materials bound to the solid phase are held in a magnetic field while unbound materials are washed away. Then, the trigger solution is added to the reaction vessel, and the light generated by the reaction is measured with the ECL100 Chemiluminescent Immunoassay Analyzer. The relative light units (RLU) are proportional to the concentration of Giardia in the sample. The amount of analyte in the sample is determined from a stored, multi-point calibration curve and reported in Units per mL concentration.
