Fecal Cryptosporidium Parvum Antigen CLIA Kit

The Fecal Cryptosporidium Parvum Antigen CLIA Kit is intended for the quantitative determination of Fecal Cryptosporidium parvum Antigen levels in stool.

Compatible with the ECL100 Chemiluminescent Immunoassay Analyzer.

For Research Use Only. Not for use in diagnostic procedures.

For Laboratory Professional Use Only.

Manufactured by Epitope Diagnostics, Inc.

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Test Principle of the Fecal Cryptosporidium Parvum Antigen CLIA Kit

The Fecal Cryptosporidium Parvum Antigen CLIA Kit is designed, developed, and produced for the quantitative measurement of Cryptosporidium parvum in stool samples. The assay utilizes a two-site “sandwich” technique with two antibodies that bind to different epitopes of Cryptosporidium parvum.

Assay calibrators, controls, or samples are added directly to a reaction vessel containing streptavidin-coated magnetic particles. An acridinium ester-conjugated antibody and a biotin-conjugated antibody are added. The magnetic particles capture the biotin antibody and an immunocomplex in the form of “magnetic particles – biotin anti-C. parvum antibody –C. parvum – acridinium ester anti-C. parvum antibody.”

The materials bound to the solid phase are held in a magnetic field while unbound materials are washed away. Then, the trigger solution is added to the reaction vessel, and the light generated by the reaction is measured with the ECL100 Chemiluminescent Immunoassay Analyzer. The relative light units (RLU) are proportional to the concentration of C. parvum in the sample. The amount of analyte in the sample is determined from a stored, multi-point calibration curve and reported in Units per mL concentration.