CRP ELISA

The CRP ELISA is an enzyme immunoassay intended for the quantitative determination of C-reactive protein in plasma, serum, stool, dried blood spots, and urine.

For research use only. Not for use in diagnostic procedures.

For Laboratory Professional Use Only.

Manufactured by Immundiagnostik AG.

Test Principle of the CRP ELISA

The CRP ELISA is a sandwich assay for the determination of CRP in serum, plasma, dried blood spots, urine, and stool samples. The wells of the microtiter plate are coated with antibodies directed against C-reactive protein.

In the first incubation step, the CRP in the samples is bound to the coated capturing antibodies (in excess). To remove all unbound substances, a washing step is carried out.

In a second incubation step, a peroxidase-labeled detection antibody is added. After another washing step to remove all unbound substances, the solid phase is incubated with the substrate, tetramethylbenzidine. An acidic stopping solution is then added. The color converts to yellow. The intensity of the yellow color is directly proportional to the concentration of CRP in the sample.

A dose-response curve of the absorbance (at 450 nm) unit vs. concentration is generated. CRP, present in the samples, is determined directly from this calibration curve.

The combination of two specific antibodies in the CRP ELISA drastically reduces the possibility of false-negative results and offers a secure system to the user.