Coenzyme Q10 HPLC Kit

SKU KCR1700 Category

$445.00

Available on backorder

Product Specifications

Method: HPLC
Sample Type (Matrix): EDTA Plasma, EDTA-whole blood, Serum
Sample Volume: 200 µl
Species: Human
Standard Range: isocratic
Size: 100 Tests
Regulatory Status: For research use only in the U.S.

Description

The Coenzyme Q10 HPLC Kit is intended for the quantitative determination of coenzyme Q10 (ubiquinone) in EDTA-whole blood, serum, and EDTA plasma.

For research use only. Not for use in diagnostic procedures.

For Laboratory Professional Use Only.

This is the RUO version of Immundiagnostik part number KC1700.

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Test Principle of the Coenzyme Q10 HPLC Kit

The first step in determining coenzyme Q10 includes sample preparation. Therefore, the internal standard is added to the calibrator, controls, and samples. Higher molecular substances are removed by precipitation and centrifugation.

The coenzyme Q10 in the supernatant is extracted by an organic solvent, evaporating afterward. The residue is resuspended in ethanol p.a. Afterward, 100 μl of the resuspended solution is injected into the HPLC system.

The separation via HPLC follows an isocratic method at 30 °C using a reversed-phase column. One run lasts 15 minutes. The chromatograms are recorded by a UV detector. The quantification is performed with the delivered calibrator; the concentration is calculated via integration of the peak areas/heights by the internal standard method.

 

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