Carbonyl Protein ELISA

The Carbonyl Protein ELISA is an enzyme immunoassay intended for the quantitative determination of protein carbonyls in human serum and plasma.

For research use only. Not for use in diagnostic procedures.

For Laboratory Professional Use Only.

This is the RUO version of Immundiagnostik part number K 7870.

Test Principle of the Carbonyl Protein ELISA

The Carbonyl Protein ELISA is designed for the quantitative determination of protein-bound carbonyls.

Reconstituted standards, controls, and derivatized samples are added into the wells of a precoated microplate. The quantification of the bound proteins is performed by adding a second antibody which is biotinylated and detected by peroxidase-labeled streptavidin. Tetramethylbenzidin (TMB) is used as a peroxidase substrate. The intensity of the color is directly proportional to the concentration of carbonyl proteins.

A dose-response curve of the absorbance unit (optical density, OD at 450 nm) vs. concentration is generated, using the values obtained from the standard. The presence of carbonyl proteins in the samples is determined directly from this curve.