The Anti-thyroid Peroxidase Antibody CLIA Kit is a Chemiluminescence Immunoassay (CLIA) intended for the quantitative measurement of Anti-thyroid peroxidase (Anti-TPO) antibody concentration in serum.
Compatible with the ECL100 Chemiluminescent Immunoassay Analyzer.
For research use only. Not for use in diagnostic procedures.
For lab professional use only.
Manufactured by Epitope Diagnostics, Inc.
Please contact us for a quote.
The Anti-thyroid peroxidase antibody CLIA Kit is designed, developed, and produced for the quantitative measurement of Anti-TPO level in serum samples. The assay utilizes aindirect method that bind to different epitopes of Anti-TPO.
Assay calibrators, controls, or serum samples are added directly to a reaction vessel together with Anti-TPO Magnetic Particle Solution (Magnetic microparticles coated with streptavidin and bound to biotinylated thyroid peroxidase protein). The magnetic particles capture thebiotinylated thyroid peroxidase protein as well as an immune-complex in the form of “magnetic particles–biotinylated thyroid peroxidase protein–Anti-TPO–Protein A- acridine ester”.
Materials bound to the solid beads are held in a magnetic field while unbound materials are washed away. Then trigger solutions are added to the reaction vessel, and light emission is measured with the ECL100 Chemiluminescent Immunoassay Analyzer. The relative light units (RLU) are proportional to the concentration of an Anti-TPO in the sample. The amount of analyte in the sample is determined from a stored, multi-point calibration curve and reported in serum Anti-TPO concentration.
