Anti ox-LDL ELISA

The Anti ox-LDL ELISA is an enzyme immunoassay intended for the quantitative determination of ox-LDL antibodies in EDTA-plasma and serum.

For research use only. Not for use in diagnostic procedures.

For research use only. Not for use in diagnostic procedures.

This is the RUO version of Immundiagnostik part number K 7809.

Test Principle of the Anti ox-LDL ELISA

The Anti ox-LDL ELISA is designed for the quantitative determination of ox-LDL antibodies.

This assay is a sandwich ELISA for the direct measurement of ox-LDL antibodies in human EDTA plasma and serum. Standards, controls, and samples containing human anti-ox-LDL antibodies are added into the wells of a microplate coated with malondialdehyde-modified low-density lipoprotein (MDA-LDL, ox-LDL).

During the first incubation period, ox-LDL immobilized on the wall of the microtiter wells captures the antibodies in the samples. After washing away the unbound components, a peroxidase-labeled conjugate is added to each microtiter well. Tetramethylbenzidine is used as a peroxidase substrate.

Finally, an acidic stop solution is added to terminate the reaction. The intensity of the yellow color is directly proportional to the ox-LDL antibody concentration of the sample. A dose-response curve of absorbance unit (optical density, OD at 450 nm) vs. concentration is generated, using the values obtained from the standard. The ox-LDL antibody concentration in the samples is determined directly from this curve