The anti-hnTG6 IgA ELISA is an enzyme immunoassay intended for the quantitative determination of anti-hnTG6 IgA in human serum and plasma.

For Research Use Only. Not for use in diagnostic procedures.

For Laboratory Professional Use Only.

This is the RUO version of Immundiagnostik item number K 9400.

Test Principle of the anti-hnTG6 IgA ELISA

The anti-hnTG6 IgA ELISA is designed for the quantitative determination of neuronal transglutaminase (TG6).

The wells of the microtiter plate are coated with TG6. At the surface of the wells, the following immunological reactions take place:

First reaction: TG6 antibodies from the sample bind to the immobilized antigen forming the antigen-antibody complex. Afterward, unbound sample components are washed from the microtiter plate.

Second reaction: A second antibody directed against human IgA and labeled with peroxidase (HRP) is added. This conjugate binds to the antigen-antibody complex. The excess conjugate is then washed from the microtiter plate.

Third reaction: The enzyme-labeled complex converts the colorless substrate into a colored (blue) product. The extent of the color development reflects the amount of TG6 antibody (IgA) present in the sample. Samples without TG6 antibodies remain colorless.

A dose-response curve of the absorbance unit (optical density, OD at 450 nm) vs. concentration is generated using the values obtained from the standard. The presence of anti-hnTG IgA in the samples is determined directly from this curve.