anti-Gliadin sIgA ELISA

The anti-Gliadin sIgA ELISA is an enzyme immunoassay intended for the determination of anti-gliadin-sIgA antibodies in stool.

For research use only. Not for use in diagnostic procedures.

For Laboratory Professional Use Only.

This is the RUO version of Immundiagnostik part number K 9311.

Test Principle of the anti-Gliadin sIgA ELISA

The antigen gliadin is immobilized on the microtiter plate. During the first incubation step, the human anti-gliadin-sIgA antibodies in the samples are bound by the immobilized antigen. After a washing step, the presence of an anti-gliadin-sIgA antibody is determined by the addition of a peroxidase-labeled anti-sIgA antibody.

Tetramethylbenzidine is used as a peroxidase substrate. The enzymatic reaction is stopped by an acidic stop solution. The absorbance of the color compound is determined photometrically at 450 nm.

The measured absorbance indicates the presence of bound anti-gliadin-sIgA antibodies. The results are evaluated by comparison with a cut-off value.