The Pepsinogen I CLIA Kit is a chemiluminescent immunoassay (CLIA) kit intended for the quantitative determination of Pepsinogen I levels in serum.
Compatible with the ECL-100 Chemiluminescent Immunoassay Analyzer.
For Research Use Only. Not for use in diagnostic procedures.
For Laboratory Professional Use Only.
Manufactured by Epitope Diagnostics, Inc.
Please contact us for a quote.
The Pepsinogen I CLIA Kit is designed, developed, and produced for the quantitative measurement of human pepsinogen I levels in serum samples. The assay utilizes a two-site “sandwich” technique with two antibodies that bind to different epitopes of pepsinogen I.
Assay calibrators, controls, or samples are added directly to a reaction vessel containing streptavidin-coated magnetic particles. Simultaneously, an acridinium ester antibody and a biotin antibody are added. The magnetic particles capture the biotin antibody and an immunocomplex in the form of “magnetic particles – biotin pepsinogen I antibody – pepsinogen I – acridinium ester pepsinogen I antibody.”
The materials bound to the solid phase are held in a magnetic field while unbound materials are washed away. Then, the trigger solution is added to the reaction vessel, and the light generated by the reaction is measured with the ECL-100 Chemiluminescent Immunoassay Analyzer. The relative light units (RLU) are proportional to the concentration of pepsinogen I in the sample. The amount of analyte in the sample is determined from a stored, multi-point calibration curve and reported in serum pepsinogen I concentration.
