The IDKmonitor® Vedolizumab Drug Level ELISA (Health Canada) is intended for the quantitative determination of free vedolizumab (therapeutic antibody against α4β7 integrin, e.g., ENTYVIO®) in EDTA plasma and serum.
Health Canada Licensed.
For Laboratory Professional Use Only.
Not for sale in the U.S. Please contact us to order or get a quote.
If you’re in the U.S., we offer an RUO version of this kit: KR9658
Manufactured by Immundiagnostik AG.
Also available:
In the first incubation step, the free vedolizumab from the sample is bound to the specific monoclonal anti-vedolizumab antibody coated on the plate. A washing step is carried out to remove all unbound substances. In a further incubation step, a Peroxidase-labeled antibody is added. Tetramethylbenzidine (TMB) is used as a substrate for peroxidase. Finally, an acidic stop solution is added to terminate the reaction.
The color changes from blue to yellow. The intensity of the yellow color is directly proportional to the concentration of free vedolizumab in the sample.
Using the values obtained from the standard, a dose-response curve of the absorbance unit (optical density, OD at 450 nm) vs. concentration is generated. This curve determines the presence of free vedolizumab in the samples directly.
