$1,050.00
Available on backorder
The Histamine Elimination Ratio (HERO) ELISA is an enzyme-linked immunosorbent assay (ELISA) intended for the quantitative measurement of serum histamine elimination.
For Research Use Only in the U.S. Not for Use in Diagnostic Procedures.
For Lab Professional Use Only.
Manufactured by Immundiagnostik AG.
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The Histamine Elimination Ratio (HERO) ELISA is designed for the quantitative determination of histamine elimination in serum. The test is based on the method of competitive enzyme-linked immunoassays.
In a first step, the serum samples are mixed with a sample buffer containing histamine, and aliquots are treated at 4 °C or 37 °C for 24 hours. The histamine concentrations of both aliquots are then measured in the ELISA.
The preparation of standards, controls, and aliquots involves adding a derivatization reagent for histamine derivatization. The prepared samples and a peroxidase-conjugated polyclonal histamine antibody are then incubated in wells of a microtiter plate coated with a histamine derivative (tracer). During the incubation period, the target histamine in the sample competes with the tracer, immobilized on the wall of the microtiter wells, for the binding of the polyclonal antibodies.
After washing away the unbound components, tetramethylbenzidine (TMB) is added as a peroxidase substrate. Finally, the enzymatic reaction is terminated by an acidic stop solution. The color changes from blue to yellow, and the absorbance is measured in a photometer at 450 nm. The intensity of the yellow color is inversely proportional to the histamine concentration in the sample; this means that high histamine concentration in the sample reduces the concentration of tracer-bound antibody and lowers the photometric signal.
A dose response curve of the absorbance unit (optical density, OD at 450 nm) vs. concentration is generated, using the values obtained from the standards. Histamine, present in the samples, is determined directly from this curve.
