$995.00
Ships within 7-10 business days.
Ships within 7-10 business days.
| Method | ELISA |
|---|---|
| Sample Type (Matrix) | Serum, EDTA Plasma |
| Sample Volume | 25 µL |
| Species | Human |
| Size | 96 wells |
| Regulatory Status | For Research Use Only. Not for use in diagnostic procedures. |
The IDKmonitor® Ustekinumab Free ADA ELISA is intended for the determination of free anti-drug antibodies (ADA) against the therapeutic TNF-alpha antibody ustekinumab (e.g. Stelara®) in EDTA plasma and serum.
For research use only. Not for use in diagnostic procedures.
For Laboratory Professional Use Only.
Manufactured by Immundiagnostik AG.
Also available:
In the first incubation step, the free anti-therapeutic antibodies from the sample are bound to the ustekinumab F(ab)2 fragments coated on the plate. A washing step is carried out to remove all unbound substances.
In a further incubation step, peroxidase-labeled ustekinumab is added. After another washing step to remove all unbound substances, the solid phase is incubated with the substrate, tetramethylbenzidine (TMB). An acidic stop solution is then added.
The color converts to yellow. The absorbance of the color compound is determined photometrically. The intensity of the color is directly proportional to the amount of bound ADAs (here: anti-ustekinumab antibodies) from the sample.
The results are evaluated by a cut-off control.
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