The Histamine ELISA Bulk Pack (Stool, 20 Plates) is intended for the quantitative determination of histamine in stool.
For research use only. Not for use in diagnostic procedures.
For Laboratory Professional Use Only.
Please contact us to order or get a quote.
Manufactured by Immundiagnostik AG.
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The Histamine ELISA is designed for the quantitative determination of histamine in stool. This assay is based on the method of competitive enzyme-linked immunoassays.
The sample preparation includes adding a derivatization reagent for histamine derivatization. Afterward, the treated samples and a peroxidase-conjugated polyclonal histamine antibody are incubated in wells of a microtiter plate coated with a histamine derivative (tracer).
During the incubation period, the target histamine in the sample competes with the tracer, immobilized on the wall of the microtiter wells, to bind the polyclonal antibodies.
After washing away the unbound components, tetramethylbenzidine (TMB) is added as a peroxidase substrate. Finally, the enzymatic reaction is terminated by an acidic stop solution. The color changes from blue to yellow, and the absorbance is measured in a photometer at 450 nm. The intensity of the yellow color is inversely proportional to the histamine concentration in the sample; this means that a high histamine concentration in the sample reduces the concentration of tracer-bound antibody and lowers the photometric signal.
Using the values obtained from the standards, a dose-response curve of the absorbance unit (optical density, OD at 450 nm) vs. concentration is generated. This curve determines the presence of histamine in the samples directly.
