Human Creatine Kinase-MB CLIA Kit (CKMB)

The Human Creatine Kinase-MB CLIA Kit is a Chemiluminescence Immunoassay (CLIA) intended for the quantitative measurement of human Creatine kinase-MB (CKMB) concentration in serum.

Compatible with the ECL100 Chemiluminescent Immunoassay Analyzer.

For research use only. Not for use in diagnostic procedures.

For lab professional use only.

Manufactured by Epitope Diagnostics, Inc.

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Test Principle of the Human Creatine Kinase-MB CLIA Kit

The Human Creatine Kinase-MB CLIA Kit is designed, developed, and produced for the quantitative measurement of human CK-MB level in serum samples. The CK-MB assay is a two-site sandwich assay to determination the level of Creatine kinase-MB.

Assay calibrators, controls, or serum samples are added directly to a reaction vessel together with magnetic particles antibody. The magnetic particles capture the CK-MB in the form of “magnetic particles–CK-MB antibody–CK-MB–acridinium ester CK-MB antibody”.

Materials bound to the solid beads are held in a magnetic field while unbound materials are washed away. Then trigger solutions are added to the reaction vessel, and light emission is measured with the ECL100 Chemiluminescent Immunoassay Analyzer. The relative light units (RLU) are proportional to the concentration of a CK-MB in the sample. The amount of analyte in the sample is determined from a stored, multi-point calibration curve and reported in serum CK-MB concentration.