The Thymosin beta-4 ELISA is an enzyme immunoassay intended for the quantitative determination of thymosin beta-4 in serum and thymus extract.

For research use only. Not for use in diagnostic procedures.

For Laboratory Professional Use Only.

Developed by Immundiagnostik AG.

We also offer a Thymosin alpha-1 ELISA.

Test Principle of the Thymosin beta-4 ELISA

The Thymosin beta-4 ELISA is designed for the quantitative determination of thymosin ß4 (Tß4) in serum and thymus preparations.

The test principle is based on a competition between antigens in the sample or standards and the antigen coated on the wells of the microplate. A peroxidase-conjugated antibody is used for detection and quantification, and tetramethylbenzidine (TMB) is used as a peroxidase substrate. The enzymatic reaction is terminated by an acidic stop solution.

The intensity of the color is inversely proportional to the concentration of Tß4. A dose-response curve of absorbance unit (optical density, OD at 450 nm) vs. concentration is generated using the values obtained from standard. Tß4, present in the samples, is determined directly from this curve.