The ImAnOx® (TAS/TAC) Kit is a photometric test system. The test is intended for the quantitative determination of the total antioxidative status/capacity (TAS/TAC) in EDTA plasma and serum.

For research use only. Not for use in diagnostic procedures.

For Laboratory Professional Use Only.

This is the RUO version of Immundiagnostik part number KC5200.

We also offer the PerOx® (TOS/TOC) Kit for the determination of the total oxidative status/capacity.

Test Principle of the ImAnOx® (TAS/TAC) Kit

The determination of the antioxidative capacity is performed by the reaction of antioxidants in the sample with a defined amount of exogenously provided hydrogen peroxide (H2O2). The antioxidants in the sample eliminate a certain amount of the provided hydrogen peroxide. The residual H2O2 is determined photometrically by an enzymatic reaction, which involves the conversion of TMB to a colored product.

After adding a stop solution, the samples are measured at 450 nm in a microtiter plate reader. The delivered calibrator performs the quantification.

The difference between applied and measured peroxide concentration in a defined time period is proportional to the reactivity of the antioxidants of the sample (antioxidative capacity). Quantification is performed with the enclosed calibrator.

Please note: As the reaction speed of the distinct antioxidants in the sample differs, the measured concentrations of antioxidants are equivalent to the reactivity of the specific antioxidants and not to their total amount in the sample. Therefore, we use hydrogen-peroxide equivalents in our test system as units for the antioxidative capacity.

The concentration of total antioxidative status/capacity (TAS/TAC) can be quantified by referring to the optical density of the calibrator.