The ImAnOx® (TAS/TAC) Kit is a photometric test system intended for the quantitative determination of the total antioxidative status/capacity (TAS/TAC) in EDTA plasma and serum.
For research use only. Not for use in diagnostic procedures.
For Laboratory Professional Use Only.
Manufactured by Immundiagnostik AG.
Also available:
The determination of the antioxidative capacity is performed by the reaction of antioxidants in the sample with a defined amount of exogenously provided hydrogen peroxide (H2O2). The antioxidants in the sample eliminate a certain amount of the provided hydrogen peroxide. The residual H2O2 is determined photometrically by an enzymatic reaction, which involves the conversion of TMB to a colored product.
After adding a stop solution, the samples are measured at 450 nm in a microtiter plate reader. The delivered calibrator performs the quantification.
The difference between applied and measured peroxide concentration in a defined time period is proportional to the reactivity of the antioxidants of the sample (antioxidative capacity). Quantification is performed with the enclosed calibrator.
The concentration of total antioxidative status/capacity (TAS/TAC) can be quantified by referring to the optical density of the calibrator.