ImAnOx® (TAS/TAC) Kit

SKU KCR5200 Category

$375.00

Ships within 7-10 business days.

Ships within 7-10 business days.

Product Specifications

MethodColorimetric
Sample Type (Matrix)EDTA Plasma, Serum
Sample Volume40 µL
SpeciesHuman
Incubation Time15m
Rangeone-point calibration
Size96 wells
Regulatory StatusFor Research Use Only. Not for use in diagnostic procedures.

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Description

The ImAnOx® (TAS/TAC) Kit is a photometric test system intended for the quantitative determination of the total antioxidative status/capacity (TAS/TAC) in EDTA plasma and serum.

For research use only. Not for use in diagnostic procedures.

For Laboratory Professional Use Only.

Manufactured by Immundiagnostik AG.

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Test Principle of the ImAnOx® (TAS/TAC) Kit

The determination of the antioxidative capacity is performed by the reaction of antioxidants in the sample with a defined amount of exogenously provided hydrogen peroxide (H2O2). The antioxidants in the sample eliminate a certain amount of the provided hydrogen peroxide. The residual H2O2 is determined photometrically by an enzymatic reaction, which involves the conversion of TMB to a colored product.

After adding a stop solution, the samples are measured at 450 nm in a microtiter plate reader. The delivered calibrator performs the quantification.

The difference between applied and measured peroxide concentration in a defined time period is proportional to the reactivity of the antioxidants of the sample (antioxidative capacity). Quantification is performed with the enclosed calibrator.

The concentration of total antioxidative status/capacity (TAS/TAC) can be quantified by referring to the optical density of the calibrator.

 

 

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