IDKmonitor® Adalimumab Free ADA ELISA

The IDKmonitor® Adalimumab Free ADA ELISA is intended for the determination of free anti-drug antibodies (ADA) against the therapeutic TNF-alpha antibody adalimumab (e.g. HUMIRA®) in EDTA plasma and serum.

For research use only. Not for use in diagnostic procedures.

For Laboratory Professional Use Only.

This is the RUO version of Immundiagnostik part number K 9652.

IDKmonitor® Resources

Download our IDKmonitor® ELISAs brochure to discover more.

Test Principle of the IDKmonitor® Adalimumab Free ADA ELISA

This is a sandwich assay for the determination of free antibodies against adalimumab (e. g. HUMIRA®). In the first incubation step, the free antitherapeutic antibodies from the sample are bound to the adalimumab F(ab)2 fragments coated on the plate. To remove all unbound substances, a washing step is carried out. In a further incubation step, peroxidase-labeled adalimumab is added. After another washing step, to remove all unbound substances, the solid phase is incubated with the substrate, Tetramethylbenzidine (TMB). An acidic stop solution is then added.

The color converts to yellow. The absorbance of the color compound is determined photometrically. The intensity of the color is directly proportional to the amount of bound ADAs (here: anti-adalimumab antibodies) from the sample. The results are evaluated by a cut-off control.