The IDK® Hemoglobin ELISA Bulk Pack (20 Plates) is intended for the quantitative determination of human hemoglobin in stool.

For research use only. Not for use in diagnostic procedures.

For Laboratory Professional Use Only.

Please inquire about pricing, as each IDK® Hemoglobin ELISA Bulk Pack is customized to meet the needs of your lab.

If your lab tests at lower volumes, the IDK® Hemoglobin ELISA is also available in a single-plate format.

Developed by Immundiagnostik AG.

Test Principle of the IDK® Hemoglobin ELISA

The IDK® Hemoglobin ELISA is used for the quantitative determination of hemoglobin in stool.

The hemoglobin in the sample is bound to anti-hemoglobin antibodies (in excess), which are immobilized on the surface of the microtiter wells. To remove all unbound substances, a washing step is carried out. In a second incubation step, an anti-hemoglobin peroxidase-labeled antibody is added. After another washing step, to remove all unbound substances, the solid phase is incubated with the substrate, tetramethylbenzidine.

An acidic solution is then added to stop the reaction. The color converts from blue to yellow. The intensity of the yellow color is directly proportional to the concentration of hemoglobin in the sample. A dose-response curve of the absorbance unit (optical density, OD) vs. concentration is generated, using the values obtained from the standard. The presence of hemoglobin in the samples is determined directly from this curve.