The Retinol-binding Protein ELISA is an enzyme immunoassay intended for the quantitative determination of free retinol-binding protein RBP/RBP4 as well as RBP4 complexed with transthyretin in plasma, serum, and urine.

For research use only. Not for use in diagnostic procedures.

For Laboratory Professional Use Only.

This is the RUO version of Immundiagnostik part number K 6110.

Test Principle of the Retinol-binding Protein ELISA

The Retinol-binding Protein ELISA is designed for the quantitative determination of retinol-binding protein RBP/RBP4 in plasma, serum, and urine.

In the first incubation step, RBP/RBP4 in the samples is bound to polyclonal rabbit anti-RBP/RBP4 antibodies immobilized on the microtitre plate. A peroxidase-conjugated anti-RBP/RBP4 antibody is used for the detection and quantification, and tetramethylbenzidine (TMB) is used as a peroxidase substrate.

A dose-response curve of absorbance unit (optical density at 450 nm) vs. concentration is generated using the values obtained from the standard. The presence of RBP/RBP4 in the samples is determined directly from this curve.