The Relaxin ELISA is an enzyme immunoassay intended for the quantitative determination of relaxin in serum, plasma, urine, seminal plasma, and tissue samples.

For research use only. Not for use in diagnostic procedures.

For Laboratory Professional Use Only.

This is the RUO version of Immundiagnostik part number K 9210.

Test Principle of the Relaxin ELISA

The Relaxin ELISA is designed for the quantitative determination of relaxin. The assay utilizes the “sandwich” technique with two selected polyclonal antibodies that bind to human Relaxin.

Assay standards, controls, and pre-diluted samples which are assayed for human Relaxin are added into the wells of a microplate coated with a high affine polyclonal anti-human Relaxin antibody.

During the first incubation step, Relaxin is bound by the immobilized antibody. Then a detection antibody, biotin-labeled anti-Relaxin, is
added.

Afterward, a peroxidase-conjugate is added into each microtiter well, and a “sandwich” of capture antibody - human Relaxin - detection antibody– peroxidase-conjugate is formed. Tetramethylbenzidine (TMB) is used as peroxidase substrate. Finally, an acidic stop solution is added to terminate the reaction.

The color changes from blue to yellow. The intensity of the yellow color is directly proportional to the concentration of Relaxin. A dose-response curve of the absorbance unit (optical density, OD at 450 nm) vs. concentration is generated using the values obtained from the standard. The presence of Relaxin in the samples is determined directly from this curve.