Malondialdehyde HPLC Kit

The Malondialdehyde HPLC Kit is intended for the quantitative determination of malondialdehyde in plasma, serum, and urine.

For research use only. Not for use in diagnostic procedures.

For Laboratory Professional Use Only.

Manufactured by Immundiagnostik AG.

Test Principle of the Malondialdehyde HPLC Kit

The first step in determining malondialdehyde is sample preparation with derivatization. The derivatization reagent transforms malondialdehyde into a fluorescent product. Afterward, the pH is optimized through the addition of a reaction solution. 20 μl of the supernatant are injected into the HPLC system.

The separation via HPLC follows an isocratic method at 30 °C using a reversed-phase column. One run lasts 4 minutes. A fluorescence detector records the chromatograms. The quantification is performed with the delivered plasma calibrator; the concentration is calculated via the external standard method’s integration of the peak heights.