LBP ELISA

The LBP ELISA is intended for the quantitative determination of lipopolysaccharide (LPS) binding protein (LBP) in human serum and EDTA plasma.

The LBP ELISA is for Research Use Only in North America. Not for use in diagnostic procedures.

For Laboratory Professional Use Only.

Developed by Immundiagnostik AG.

Test Principle of the LBP ELISA

The LBP ELISA is designed for the quantitative determination of LBP in serum and plasma samples. Standards and prepared samples assayed for LBP are added to the wells of a microplate coated with a high affine anti-LBP antibody.

During the first incubation step, LBP from the samples is bound to the primary antibody coated to the microtiter plate. Then a detection antibody, a biotin-labeled anti-LBP antibody, is added. Afterward, a peroxidase-conjugate is added into each microtiter well, and a “sandwich” of capture antibody– LBP – biotinylated antibody – streptavidin-peroxidase conjugate is formed. Tetramethylbenzidine (TMB) is used as peroxidase substrate.

Finally, an acidic stop solution is added to terminate the reaction. The color changes from blue to yellow. The intensity of the yellow color is directly proportional to the concentration of LBP. A dose-response curve of the absorbance unit (optical density, OD at 450 nm) vs. concentration is generated using the values obtained from the standard. LBP, present in the samples, is determined directly from this curve.