Immunoglobulin E ELISA

The Immunoglobulin E ELISA is an enzyme immunoassay intended for the quantitative determination of IgE in serum and stool.

For research use only. Not for use in diagnostic procedures.

For Laboratory Professional Use Only.

This is the RUO version of Immundiagnostik part number K 6511.

Test Principle of the Immunoglobulin E ELISA

The Immunoglobulin E ELISA is designed for the quantitative determination of IgE. In the first incubation step, the IgE in the samples is bound to polyclonal antibodies (in excess), and immobilized to the microtiter wells’ surfaces. To remove all unbound substances, a washing step is carried out.

In a second incubation step, a peroxidase-labeled anti-IgE (POD-antibody) antibody (polyclonal) is added. After another washing step to remove all unbound substances, the solid phase is incubated with the substrate, Tetramethylbenzidine (TMB). An acidic stop solution is then added to stop the reaction. The color converts from blue to yellow. The intensity of the yellow color is directly proportional to the concentration of IgE.

A dose-response curve of the absorbance unit (optical density, OD at 450 nm) vs. concentration is generated using the values obtained from the standard. IgE, present in the samples, is determined directly from this curve.