The IDK® Zonulin ELISA Bulk Pack (Stool, 20 Plates) is intended for the quantitative determination of zonulin family peptides (ZFP) in stool.

For research use only. Not for use in diagnostic procedures.

For Laboratory Professional Use Only.

Please inquire for pricing, as each IDK® Zonulin ELISA Bulk Pack is customized to meet the needs of your lab.

Test Principle of the IDK® Zonulin ELISA Bulk Pack

The IDK® Zonulin ELISA (Stool) is based on the method of competitive ELISA.

As a first preparation step, biotinylated ZFP is added to the samples, standards, and controls. Afterward, aliquots of the treated samples, standards, and controls are transferred and incubated in microtiter plate wells coated with polyclonal anti-ZFP antibodies. During the incubation, the free target antigen in the samples competes with the biotinylated ZFP for the binding of the polyclonal anti-ZFP antibodies immobilized on the microtiter plate wells. The unbound components are removed by a washing step.

During a second incubation step, peroxidase-labeled streptavidin, which binds to the biotinylated ZFP, is added to each microtiter well. After a washing step to remove the unbound components, the peroxidase substrate tetramethylbenzidine is added.

Finally, the enzymatic reaction is terminated by an acidic stop solution. The color changes from blue to yellow, and the absorbance is measured in the photometer at 450 nm. The intensity of the yellow color is inversely proportional to the ZFP concentration in the sample; this means a high ZFP concentration in the sample reduces the concentration of the biotinylated ZFP bound to the immobilized anti-ZFP antibodies and lowers the photometric signal.

A dose-response curve of absorbance unit (optical density, OD at 450 nm) vs. concentration is generated using the values obtained from the standard.