IDK® Hemoglobin / Haptoglobin Complex ELISA

The IDK® Hemoglobin/Haptoglobin Complex ELISA is an enzyme immunoassay intended for the quantitative determination of human hemoglobin/haptoglobin complex in stool.

For research use only. Not for use in diagnostic procedures.

For Laboratory Professional Use Only.

The IDK® Hemoglobin/Haptoglobin Complex ELISA is compatible with our IDK Extract® Stool Sample Preparation System.

This is the RUO version of Immundiagnostik part number K 7817D.

Test Principle of the IDK® Hemoglobin/Haptoglobin Complex ELISA

The IDK® Hemoglobin/Haptoglobin Complex ELISA is designed for the quantitative determination of hemoglobin/haptoglobin complex in stool.

The complex in the sample is bound to anti-haptoglobin antibodies (in excess), which are immobilized on the surface of the microtiter wells. To remove all unbound substances, a washing step is carried out.

In a second incubation step, an anti-hemoglobin peroxidase-labeled antibody is added. After another washing step to remove all unbound substances, the solid phase is incubated with a tetramethylbenzidine (TMB) substrate. An acidic solution is then added to stop the reaction. The color converts from blue to yellow. The intensity of the yellow color is directly proportional to the concentration of hemoglobin/haptoglobin complex in the sample.

A dose-response curve of the absorbance unit (optical density, OD) vs. concentration is generated using the values obtained from the standard. The presence of hemoglobin/haptoglobin complex in the samples is determined directly from this curve.