The IDK® anti-htTG sIgA ELISA is an enzyme immunoassay intended for the quantitative determination of anti-human tissue Transglutaminase sIgA antibodies in stool.

For research use only. Not for use in diagnostic procedures.

For Laboratory Professional Use Only.

The IDK® anti-htTG sIgA ELISA (Stool) is compatible with our IDK Extract® Stool Sample Preparation System.

This is the RUO version of Immundiagnostik part number K 9393.

Test Principle of the IDK® anti-htTG sIgA ELISA

The IDK® anti-htTG sIgA ELISA is designed for the quantitative determination of anti-htTG sIgA antibodies in feces.

In the first incubation step, the anti-htTG sIgA antibodies in the sample are bound to their antigen (human recombinant transglutaminase), which is immobilized on the surface of the microtiter plates. To remove all unbound foreign substances, a washing step is carried out.

In a second incubation step, a peroxidase-labeled sIgA antibody is added. After another washing step, to remove all unbound antibodies, the solid phase is incubated with the substrate, tetramethylbenzidine.

An acidic stop solution is then added to stop the reaction. The color converts from blue to yellow. The intensity of the yellow color is directly proportional to the amount of bound antibodies and can be determined photometrically at 450 nm. The results are evaluated by comparison with a cut-off value.