beta-2-Microglobulin ELISA

The beta-2-Microglobulin ELISA is an enzyme immunoassay intended for the quantitative determination of ß2-microglobulin in serum, plasma, and urine.

For research use only. Not for use in diagnostic procedures.

For Laboratory Professional Use Only.

This is the RUO version of Immundiagnostik part number K 6210.

Test Principle of the beta-2-Microglobulin ELISA

In the first incubation step, ß2-microglobulin is bound to an immobilized antibody. Then a peroxidase-labeled anti-ß2-microglobulin-antibody is added, and a sandwich of capture antibody – ß2-microglobulin – peroxidase-conjugate is formed.

Tetramethylbenzidine (TMB) is used as a peroxidase substrate. Finally, an acidic stop solution is added to terminate the reaction. The color changes from blue to yellow. The intensity of the color is directly proportional to the concentration of ß2-microglobulin.

A dose-response curve of the absorbance unit (optical density, OD at 450 nm) vs. concentration is generated using the values obtained from the standards. ß2-microglobulin, present in the samples, is determined directly from this curve.