alpha-1-Microglobulin ELISA

SKU KR6710 Category

$285.00

Product Specifications

Method: ELISA
Sample Type (Matrix): Serum, Plasma, Urine
Sample Volume: 10 µl
Species: Human
Incubation time: 2h 15m
Standard Range: 0.019-1.5 mg/l
Size: 96 wells
Regulatory Status: For research use only in the U.S.

Description

The alpha-1-Microglobulin ELISA is an enzyme immunoassay intended for the quantitative determination of alpha-1-microglobulin in serum, plasma, and urine.

For research use only. Not for use in diagnostic procedures.

For Laboratory Professional Use Only.

This is the RUO version of Immundiagnostik part number K 6710.

Test Principle of the alpha-1-Microglobulin ELISA

The alpha-1-Microglobulin ELISA is designed for the quantitative determination of α1-microglobulin in serum, plasma, and urine.

The α1-microglobulin in the samples is bound to an excess of polyclonal rabbit anti-α1-microglobulin antibodies immobilized to the surface of the microtitre plate. After a washing step to remove all foreign substances, the quantification of the bound α1-microglobulin is carried out by adding a peroxidase-labeled antibody, which also binds to the α1-microglobulin. The amount of converted peroxidase substrate is directly proportional to the amount of bound α1-microglobulin.

A dose-response curve of the absorbance unit (optical density, OD at 450 nm) vs. concentration is generated using the values obtained from the standard. The presence of α1-microglobulin in the samples is determined directly from this curve.

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