Albumin ELISA

The Albumin ELISA is an enzyme immunoassay intended for the quantitative determination of albumin in urine and stool.

The Albumin ELISA is compatible with our IDK Extract® Stool Sample Preparation System.

For research use only. Not for use in diagnostic procedures.

For Laboratory Professional Use Only.

This is the RUO version of Immundiagnostik AG part number K 6330.

Test Principle of the Albumin ELISA

The Albumin ELISA is a two-step assay for the ultrasensitive determination of human albumin in stool and urine. A polyclonal rabbit antibody specific for human albumin is immobilized on a microtiter plate, and a second anti-albumin antibody is conjugated to peroxidase.

In the first incubation step, the albumin in the samples is bound to the immobilized anti-albumin antibodies. A washing step is carried out to remove all unbound substances. In a second incubation step, a peroxidase-labeled anti-albumin antibody is added. After another washing step to remove all unbound substances, a peroxidase substrate, tetramethylbenzidine, is added.

The enzymatic reaction is terminated by an acidic stop solution, whereby the color converts from blue to yellow. The intensity of the yellow color is directly proportional to the concentration of albumin in the sample.

A dose-response curve of the absorbance unit (optical density, OD at 450 nm) vs. concentration is generated using the values obtained from the standard. The presence of albumin in the samples is determined directly from this curve.