The ADMA Xpress ELISA is intended for the quantitative determination of asymmetric dimethyl arginine (ADMA) in serum, citrate, and EDTA plasma.

For research use only. Not for use in diagnostic procedures.

For Laboratory Professional Use Only.

This is the RUO version of Immundiagnostik part number K 7860.

Test Principle of the ADMA Xpress ELISA

The ADMA Xpress ELISA is designed for the quantitative determination of ADMA. This assay is based on the method of competitive enzyme-linked immunoassays.

The sample preparation includes the addition of a derivatization reagent for ADMA derivatization, and a reaction buffer is added containing ADMA-derivative (tracer). Afterward, the treated samples are incubated in the wells of a microtiter plate coated with a polyclonal antibody against ADMA-derivative. During the incubation period, the target ADMA in the sample competes with the tracer for the binding of the polyclonal antibodies, immobilized on the wall of the microtiter wells.

During the second incubation step, a peroxidase conjugate is added to each microtiter well to detect the tracer. After washing away the unbound components, tetramethylbenzidine (TMB) is added as a peroxidase substrate.

Finally, the enzymatic reaction is terminated by an acidic stop solution. The color changes from blue to yellow, and the absorbance is measured in a photometer at 450 nm. The intensity of the yellow color is inversely proportional to the ADMA concentration in the sample; this means, high ADMA concentration in the sample reduces the concentration of antibody-bound tracer and lowers the photometric signal.

A dose-response curve of the absorbance unit (optical density, OD at 450 nm) vs. concentration is generated, using the values obtained from the standards. The presence of ADMA in the samples is determined directly from this curve.