The Calprotectin Mouse/Rat ELISA Kit is intended for the quantitative determination of S100A8/S100A9 (MRP 8/14) in stool, serum, plasma, urine, tissue extract, and cell culture supernatant.

The Calprotectin Mouse/Rat ELISA is for research use only. Not for use in diagnostic procedures.

For Laboratory Professional Use Only.

Developed by Immundiagnostik AG.

Test Principle of the Calprotectin Mouse/Rat ELISA

The assay utilizes the two-site sandwich technique with two selected antibodies that bind to S100A8/S100A9. Standards, controls, and diluted samples that are assayed for S100A8/S100A9 are added to the microtiter wells coated with high-affinity anti-S100A8/S100A9 antibodies.

During the first incubation step, S100A8/S100A9 in the samples is bound by the immobilized antibodies. In the next incubation step, a monoclonal anti-S100A8/S100A9 antibody is added to each microtiter well. Then a peroxidase-labeled antimouse conjugate is pipetted into each well, and the following complex is formed: capture antibodies – S100A8/S100A9 – detection antibody – peroxidase conjugate.

Tetramethylbenzidine is used as a substrate for peroxidase. Finally, an acidic stop solution is added to terminate the reaction. The color changes from blue to yellow. The intensity of the yellow color is directly proportional to the S100A8/S100A9 concentration of the sample.

A dose-response curve of the absorbance unit (optical density, OD at 450 nm) vs. concentration is generated, using the values obtained from the standard. S100A8/S100A9 present in the samples is determined directly from this curve.