IDK® alpha-1-Antitrypsin ELISA (1-point-calibration)

The IDK® alpha-1-Antitrypsin ELISA is an enzyme immunoassay intended for the quantitative determination of alpha-1-Antitrypsin in stool.

For research use only. Not for use in diagnostic procedures.

For Laboratory Professional Use Only.

This is the RUO version of Immundiagnostik part number K 6760.

Test Principle of the IDK® alpha-1-Antitrypsin ELISA

This test is designed for the quantitative determination of α1-Antitrypsin in stool. The assay utilizes the sandwich technique with two selected antibodies that bind to human α1-antitrypsin. Calibrator, controls, and prediluted samples assayed for human α1-antitrypsin are added to the wells of a microplate coated with a high affine anti-human α1-antitrypsin antibody.

During the first incubation step, α1-antitrypsin is bound by the immobilized antibody. Then a peroxidase-conjugated polyclonal anti-human α1-antitrypsin antibody is added into each microtiter well, and a “sandwich” of capture antibody – human α1-antitrypsin – peroxidase-conjugate is formed. Tetramethylbenzidine (TMB) is used as a peroxidase substrate.

Finally, an acidic stop solution is added to terminate the reaction. The color changes from blue to yellow. The intensity of the yellow color is directly proportional to the concentration of α1-antitrypsin. Samples are quantified by referring their optical density to a lot-dependent master calibration curve and using a calibrator run with each test.