25-OH Vitamin D3/D2 RP-HPLC Kit
Available with Lead Time!
Sample Type (Matrix): Serum, Plasma
Sample Volume: 500 µl
Standard Range: isocratic
Size: 100 Tests
Regulatory Status: For research use only in the U.S.
Kit Manual: Download
The 25-OH Vitamin D3/D2 RP-HPLC Kit is intended for the quantitative determination of 25-OH vitamin D3 and 25-OH vitamin D2 in serum and plasma.
For research use only. Not for use in diagnostic procedures.
For Laboratory Professional Use Only.
This is the RUO version of Immundiagnostik part number KC3000.
Browse our full offering of Vitamin D testing tools.
Test Principle of the 25-OH Vitamin D3/D2 RP-HPLC Kit
To determine 25-OH vitamin D3 and 25-OH vitamin D2 samples, simple sample preparation is used, a combination of precipitation and extraction.
The HPLC separation works with an isocratic method at 30 °C with a "reversed phase" column. Chromatograms are detected by a UV detector. The separation takes 12 minutes for each run. Results are quantified by the provided calibrator and calculated by the "external standard method" by determination of the peak height.
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